What should be used on a cultured CVS sample to obtain more slides for analysis?

What should be used on a cultured CVS sample to obtain more slides for analysis?

• A. Colchicine
• B. Trypsin-EDTA
• C. EDTA only
• D. Acetic acid

Answer: (B)

In the context of preparing cultured Chorionic Villus Sampling (CVS) samples for cytogenetic analysis, trypsin-EDTA is utilized effectively to produce more slides. This solution serves multiple purposes in the process of cell culture and slide preparation. Trypsin, an enzyme, facilitates the dissociation of adherent cells by breaking down proteins in the extracellular matrix and cell surface. This is critical when working with cultured cells, as it allows for proper harvesting without causing damage to the cells themselves. EDTA, on the other hand, chelates calcium ions, which are important for cell adhesion, further aiding in the detachment process. Together, trypsin-EDTA ensures that cells are dispersed efficiently, allowing for increased availability of cells for slide preparation. This combination is preferred for generating slides because it allows for a higher yield of viable cells compared to using just EDTA alone or other solutions. The effectiveness of trypsin-EDTA in the detachment process maximizes the number of usable cells and contributes to higher-quality results in subsequent analyses.

In the context of preparing cultured Chorionic Villus Sampling (CVS) samples for cytogenetic analysis, trypsin-EDTA is utilized effectively to produce more slides. This solution serves multiple purposes in the process of cell culture and slide preparation.

Trypsin, an enzyme, facilitates the dissociation of adherent cells by breaking down proteins in the extracellular matrix and cell surface. This is critical when working with cultured cells, as it allows for proper harvesting without causing damage to the cells themselves. EDTA, on the other hand, chelates calcium ions, which are important for cell adhesion, further aiding in the detachment process. Together, trypsin-EDTA ensures that cells are dispersed efficiently, allowing for increased availability of cells for slide preparation.

This combination is preferred for generating slides because it allows for a higher yield of viable cells compared to using just EDTA alone or other solutions. The effectiveness of trypsin-EDTA in the detachment process maximizes the number of usable cells and contributes to higher-quality results in subsequent analyses.